Flp-in 293
WebJan 1, 2012 · We described here a simple method to display full-length antibody on Flp-In 293 cells (F293) by transient transfection of a single vector into the cells. The full-length antibodies can easily be detected on cell surface by flow cytometry 36–48 h post-transfection. 2 Materials. WebThe human embryonic kidney or HEK 293 cell line was derived from human embryonic kidney cells grown in tissue culture. The cell line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA. A portion of human adenovirus 5, nucleotides (nts) 1-4344, is integrated into chromosome 19 of the HEK cells.
Flp-in 293
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WebFlp In 293 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebFlp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells … Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
WebApr 6, 2024 · As most GBA1 mutations lead to a misfolded enzyme that is rapidly degraded via ER-associated degradation by the proteasome 11,12, we generated stable Flp-In™T-REx™-HEK 293 cell lines ... Webjor components of the Flp-In™ T-REX™ System include: • A Flp-In™ target site vector, pFRT/lacZeo, for generation of a Flp-In™ host cell line containing an integrated FRT site. …
WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In-293 cell line was created by transfecting the parent cell ... WebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster …
WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted …
WebThe Flp-In-293 and Flp-In-CV-1 cell lines contain a single integrated FRT site and stably express the lacZ-Zeocin fusion gene from the pFRT/lacZeo plasmid under the control of … citibank credit card for studentsWebDec 1, 2024 · Results: Epimagnolin A inhibited calcein export by Flp-In-293/ABCB1 cells in a concentration-dependent manner in a calcein assay. ATPase assay revealed a concentration-dependent stimulation of the ATPase activity of ABCB1 by epimagnolin A. Epimagnolin A also showed saturation kinetics in the relationship between the compound … citibank credit card formsWebApr 7, 2024 · Flp-in-T-REx 293 cells expressing either GFP or 3xFlag-tagged human SRRM4 were induced with 1 ug/ml for 24 h . Total RNA was extracted using the Illustra RNAspin Mini Isolation kit (GE Healthcare). Total RNA was extracted using the Illustra RNAspin Mini Isolation kit (GE Healthcare). dianthus beauties rebeccaWebLanguage Label Description Also known as; English: Flp-In-293. cell line dianthus biotechWebMethods: The human P-gp (ABCB1/Flp-In™-293) stable expression cells were established by using the Flp-In™ system. The effects of tenulin and isotenulin on cell viability were evaluated by SRB assays in established cell lines, sensitive cancer cell line (HeLaS3), and resistant cancer cell line (KB-vin). ... citibank credit card forgot passwordWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. citibank credit card for kidsWebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … dianthus bicolor