T4 ligase promega

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August undefined, 2024

WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. Web1.5ml Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent …

T4 DNA Ligase (HC) from Promega Biocompare.com

WebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme … WebDNA Ligase Selection Chart While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart. jordan fabrics binding tutorial

T4 DNA Ligase NEB

WebT4 DNA Ligase 1–3u/µl/100u (Weiss units) $ 55.00 Your price: Log in T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´ … Web• 1 μL of T4 DNA ligase (Promega) • x μL water up to 20 μL total. *BsaI-HF is optimized for different buffer conditions and doesn't work well ** Promega T4 Ligase buffer has been shown to offer the highest activity for BsaI enzyme. Other T4 ligase enzymes will work, but Promega enzyme with Promega buffer is highest efficiency. how to interpret the slope

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Global Ligase Market 2024-2029

WebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. WebT4 RNA Ligase 一本鎖RNAまたは一本鎖DNAの5'-末端のリン酸基と3'-末端の水酸基をATP依存的に結合 M1051 関連製品 T4 Polynucleotide Kinase DNAシークエンシング … how to interpret the regression analysisWebApr 24, 2009 · Restriction endonucleases and T4 DNA ligase were obtained from Promega. Genomic DNA of B. cereus was purified as described in reference 16. Plasmid DNA was purified using anion-exchange columns (Promega). PCR amplification of DNA was carried out with Taq polymerase (Roche Molecular Biochemicals). how to interpret the mean

"WebPromega Corporation · 2800 Woods Hollow Road·Madison, WI 53711-5399 U.S.A. ·Toll Free in the USA 800-356-9526 ·Telephone 608-274-4330 ·Internet www.promega.com Usage Information I. Description T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a … " - T4 ligase promega

T4 ligase promega

T4 DNA Ligase Buffer - Thermo Fisher Scientific

Web小鼠精胺氧化酶基因SMO启动子的结构和功能研究.pdf WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP.

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WebT4 DNA Ligase, Promega; ... T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a … WebT4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme.

WebFAQ. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single ... WebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes. Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (15)

WebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from … WebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes. Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (64)

WebT4 RNA Ligaseは、ATPに依存して、一本鎖RNAまたはDNAの分子間および分子内の連結を行い、一本鎖RNAまたはDNAの5′-末端のリン酸基と3′-末端の水酸基を結合します。同様に、一本鎖RNAの3’-末端に[5′-32P]Cytidine 3', 5'-bis(phosphate) (pCp)を付加する反応も行います。本酵素はRNase Iを欠如する組換えE.coli CA4 ...

WebNEB T4 DNA Ligase ( NEB #M0202) is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using 0.1-1 … how to interpret the regression lineWebAccidentally we have a large amount of Sigma-Aldrich DNA Ligase from T4-infected Escherichia coli and Promega T4 DNA ligase buffer. The "correct" Sigma-Aldrich buffer is made by 500 mM Tris-HCl ... jordan fabrics disappearing pinwheelWeb2X Rapid Ligation Buffer. 1.5ml. Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. Ligate DNA inserts into plasmid vectors in as little as 15 minutes. No purification necessary prior to transformation of ... how to interpret thermal imagesWebApr 13, 2024 · The resulting monophosphate RNA is intramolecularly circularized using T4 RNA ligase to produce a circular RNA with the 5′ untranslated regions (UTR) fused to the 3′ end polyA tail followed by ... jordan fabrics tradewinds patternWebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant Escherichia coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. Revised 4/18. Complete Protocol PDF (113k) how to interpret the move indexWebPromega T4 DNA Ligase. For ligation of DNA ends. Manufacturer: Promega M1804. View more versions of this product. Catalog No. PR-M1804. $278.50 / Each of 1. Qty Check … how to interpret the mean in spssWebDNA Ligase Master Mixes are pre-mixed, ready-to-use formulations that are specially formulated for Blunt/TA or Sticky Ends. The tool NEBCloner can help you select the best ligase formulation for your needs. The following tips will help to achieve maximum results from your ligation reactions. Reaction Buffers. T4 DNA Ligase Buffer should be ... how to interpret the pareto chart